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iscript tm cdna synthesis kit  (Bio-Rad)


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    Bio-Rad iscript tm cdna synthesis kit
    Iscript Tm Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 6234 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iscript tm cdna synthesis kit/product/Bio-Rad
    Average 99 stars, based on 6234 article reviews
    iscript tm cdna synthesis kit - by Bioz Stars, 2026-03
    99/100 stars

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    ( A ) Experimental design. ( B ) Viral detection by qPCR assay 48 h post-infection (48 hpi). Results are expressed as relative units per 500 ng of RNA used to produce cDNA and are representative of two different experiments ( n = 5). ( C ) Representative images of lung sections stained by hematoxylin and eosin (mock group and 48hpi) (scale bar 200 μm and 50 μm). ( D ) Histopathological scores in lung sections ( n = 5) of BALB/c WT and AnxA1KO mice. ( E ) Vascular leakage evaluated by Evans Blue concentration assay in the lungs. Concentration of TNF, IL-6, and CXCL1 in the lungs (F, G, and H, respectively) (expressed as pg/ml of homogenized tissue supernatant) ( n = 5). ( H ) *** P <0.01 (WT vs. AnxA1KO 48hpi). ** P <0.0001 (WT vs. AnxA1KO 48hpi in B and G). n.s.: not significant. In ( B ), Mann–Whitney test was used to assess the differences between the groups. From ( D ) to ( H ), two-way ANOVA tests followed by Tukey’s multiple comparison test were used to assess the differences between the groups. Results are expressed as mean ± SEM. AnxA1, annexin A1; KO, knockout.

    Journal: Clinical Science (London, England : 1979)

    Article Title: Annexin A1 as a key modulator of lung inflammation during coronavirus infections

    doi: 10.1042/CS20255801

    Figure Lengend Snippet: ( A ) Experimental design. ( B ) Viral detection by qPCR assay 48 h post-infection (48 hpi). Results are expressed as relative units per 500 ng of RNA used to produce cDNA and are representative of two different experiments ( n = 5). ( C ) Representative images of lung sections stained by hematoxylin and eosin (mock group and 48hpi) (scale bar 200 μm and 50 μm). ( D ) Histopathological scores in lung sections ( n = 5) of BALB/c WT and AnxA1KO mice. ( E ) Vascular leakage evaluated by Evans Blue concentration assay in the lungs. Concentration of TNF, IL-6, and CXCL1 in the lungs (F, G, and H, respectively) (expressed as pg/ml of homogenized tissue supernatant) ( n = 5). ( H ) *** P <0.01 (WT vs. AnxA1KO 48hpi). ** P <0.0001 (WT vs. AnxA1KO 48hpi in B and G). n.s.: not significant. In ( B ), Mann–Whitney test was used to assess the differences between the groups. From ( D ) to ( H ), two-way ANOVA tests followed by Tukey’s multiple comparison test were used to assess the differences between the groups. Results are expressed as mean ± SEM. AnxA1, annexin A1; KO, knockout.

    Article Snippet: The cDNA was synthesized with 500 ng of total RNA using the iScript TM gDNA Clear cDNA Synthesis Kit (BIO-RAD) following manufacturer’s instructions.

    Techniques: Infection, Staining, Concentration Assay, MANN-WHITNEY, Comparison, Knock-Out